Rheinheimera longhuensis sp. nov., isolated from a slightly alkaline lake in northeast of China and emended description of genus Rheinheimera Brettar et al. 2002 emend. Merchant et al. 2007 emend. Chen et al. emend. Li et al. 2011.
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Address: Institute of Microbiology, Chinese Academy of Sciences;
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The bacterial strain LH2-2T was isolated from freshwater of Longhu lake, a slightly alkaline lake (pH 8.8), in northeast of China. Cells of strain LH2-2T were Gram-staining negative, non-spore-forming rods measuring 0.3-0.5 μm in width and 2.0-4.0 μm in length. Cells were motile by means of a single polar flagellum. Strictly aerobic and heterotrophic. Oxidase- and catalase-positive. Growth occurs at 0-36 °C (optimum, 26-34°C), pH 6.5-11 (optimum, pH 8.0-8.6) and in the presence of 0-2 % NaCl (w/v, optimum, 1%). Strain LH2-2T contained Q-8 as the major respiratory quinine. The major fatty acids were summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH, 21.9 %), C17:1ω8c (18.9%), C18:1ω7c (16.4%) and C16:0 (12.7%) when it was grown on marine agar 2216. DNA G+C content was 47 mol% (Tm). The 16S rRNA gene and a conserved portion of gyrase B gene (gyrB) were sequenced and used for phylogenetic analyses. Phylogenetic trees based on 16S rRNA gene and gyrB sequences showed that strain LH2-2T was associated with the genus Rheinheimera and closely related to the type strains of Rheinheimera species, and showed highest 16S rRNA gene sequence similarity to R. pacifica KMM 1406T (97.4 %), and then to R. aquimaris SW-353T (97.1 %) and R. chironomi K19414T (96.5 %). The DNA-DNA relatedness of strain LH2-2T to R. pacifica KMM 1406T, R. aquimaris SW-353T and R. chironomi K19414T was 39, 31 and 23 %, respectively. Based on these results, it is concluded that strain LH2-2T represents a novel species of the genus Rheinheimera, for which the name Rheinheimera longhuensis sp. nov. is proposed. The type strain is LH2-2T (=CGMCC 1.7003T= NBRC 105632T).
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