Outer membrane protein OprF is the major outer membrane of Pseudomonas aeruginosa, and has been expressed to a similar high level in Escherichia coli from the cloned gene. It contains conserved surface epitopes, and antibodies against these epitopes can protect mice from P. aeruginosa infections. To develop the oprF gene as a carrier for foreign epitopes, linker insertion mutagenesis has been performed to introduce 12 nucleotide inserts marked by a unique PstI site. Nine such sites can accept and express a foreign epitope within the surface loop regions of OprF on the surface of E. coli. The antigenicity at a given insertion site, and the influence of the length of a model repeating malarial epitope on antigenicity, have been shown to be site-specific and apparently dependent on the nature of the surrounding amino acids at the insertion site. Immunization of mice with OprF containing a highly antigenic inserted epitope led to an epitope-specific antibody response. These data suggest that OprF has Potential for use as a carrier for foreign epitopes.
Related ArticlesThe effect of the length of a malarial epitope on its antigenicity and immunogenicity in an epitope presentation system using the Pseudomonas aeruginosa outer membrane protein OprF as the carrier.FEMS Microbiol Lett. 1996
The effect of the length of a malarial epitope on its antigenicity and immunogenicity in an epitope presentation system using the Pseudomonas aeruginosa outer membrane protein OprF as the carrier.
Wong RS, Hancock RE. FEMS Microbiol Lett. 1996 Jul 1; 140(2-3):209-14.
Protection against P. aeruginosa with an adenovirus vector containing an OprF epitope in the capsid.J Clin Invest. 2005
Protection against P. aeruginosa with an adenovirus vector containing an OprF epitope in the capsid.
Worgall S, Krause A, Rivara M, Hee KK, Vintayen EV, Hackett NR, Roelvink PW, Bruder JT, Wickham TJ, Kovesdi I, et al. J Clin Invest. 2005 May; 115(5):1281-9. Epub 2005 Apr 1.
Pseudomonas aeruginosa outer membrane protein OprF as an expression vector for foreign epitopes: the effects of positioning and length on the antigenicity of the epitope.Gene. 1995
Pseudomonas aeruginosa outer membrane protein OprF as an expression vector for foreign epitopes: the effects of positioning and length on the antigenicity of the epitope.
Wong RS, Wirtz RA, Hancock RE. Gene. 1995 May 26; 158(1):55-60.
Review Pseudomonas aeruginosa antigens as potential vaccines.FEMS Microbiol Rev. 1997
Review Pseudomonas aeruginosa antigens as potential vaccines.
Stanislavsky ES, Lam JS. FEMS Microbiol Rev. 1997 Nov; 21(3):243-77.
Review PhoE protein as a carrier for foreign epitopes.Int Rev Immunol. 1994
Review PhoE protein as a carrier for foreign epitopes.
Janssen R, Tommassen J. Int Rev Immunol. 1994; 11(2):113-21.
Safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein F-I vaccine in human volunteers.Infect Immun. 1999
Safety and immunogenicity of a Pseudomonas aeruginosa hybrid outer membrane protein F-I vaccine in human volunteers.
Mansouri E, Gabelsberger J, Knapp B, Hundt E, Lenz U, Hungerer KD, Gilleland HE Jr, Staczek J, Domdey H, von Specht BU. Infect Immun. 1999 Mar; 67(3):1461-70.
Pseudomonas aeruginosa fur overlaps with a gene encoding a novel outer membrane lipoprotein, OmlA.J Bacteriol. 1999
Pseudomonas aeruginosa fur overlaps with a gene encoding a novel outer membrane lipoprotein, OmlA.
Ochsner UA, Vasil AI, Johnson Z, Vasil ML. J Bacteriol. 1999 Feb; 181(4):1099-109.
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