pH-sensitive capsules as intracellular optical reporters for monitoring lysosomal pH changes upon stimulation.
Authors: Gil PR,Nazarenus M,Ashraf S,Parak WJ,
Address: Fachbereich Physik and WZMW, Philipps Universität Marburg, Renthof 7, D-35037, Marburg, Germany.
Publication: 2012 Mar 26;8(6):943-8. doi: 10.1002/smll.201101780. Epub 2012 Feb 8.
The concept of a long-term sensor for ion changes in the lysosome is presented. The sensor is made by layer-by-layer assembly of oppositely charged polyelectrolytes around ion-sensitive fluorophores, in this case for protons. The sensor is spontaneously incorporated by cells and resides over days in the lysosome. intracellular changes of the concentration of protons upon cellular stimulation with pH-active agents are monitored by read-out of the sensor fluorescence at real time. With help of this sensor concept it is demonstrated that the different agents used (Monensin, Chloroquine, Bafilomycin A1, Amiloride) possessed different kinetics and mechanisms of action in affecting the intracellular pH values.
Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
The contents within traveldoctoronline are presented only for informational purposes and cannot substitute for professional health care or any other medical treatment.All users of this website with health problems should be oblige always to consult their medical doctor before starting any treatment.