Pax6 expression during retinogenesis in sharks: comparison with markers of cell proliferation and neuronal differentiation.

Authors:
Address: Department of Cell Biology and Ecology, University of Santiago de Compostela, Santiago de Compostela, Spain.
Journal:

Publication:

abstract

Pax6 is a highly conserved transcription factor that appears involved in the entire process of retinogenesis, including maintenance of proliferation of retinal progenitors and differentiation of particular neuron fates. To gain insight into the retinogenesis in fish, we study the dynamics of Pax6 expression in the developing and mature retina of two sharks that inhabit in particular environments, and compare it with the dynamics of a marker of cell proliferation (proliferating cell nuclear antigen, PCNA) and markers of neuronal differentiation, such as glutamic acid decarboxylase (GAD), calretinin (CR), tyrosine-hydroxylase, and serotonin (5-HT). Our results reveal that Pax6 is expressed in PCNA-immunoreactive cells within the nonlayered retina, suggesting a role for Pax6 in proliferating progenitors. Pax6 expression decays as development proceeds and eventually remains in some postmitotic cells, which points to additional roles of Pax6 following neurogenesis. Double immunofluorescence reveals Pax6/CR colocalization in the ganglion cell layer, Pax6/5-HT in the inner part of the inner nuclear layer (INLi), and Pax6/GAD in the INLi and horizontal cell layer. Our results suggest that Pax6 may contribute to neuron diversification in the neural retina. J. Exp. Zool. (Mol. Dev. Evol.) 314B, 2011. © 2011 Wiley Periodicals, Inc.

Related Articles
Pax6 expression during retinogenesis in sharks: comparison with markers of cell proliferation and neuronal differentiation.
J Exp Zool B Mol Dev Evol. 2012
Pax6 expression during retinogenesis in sharks: comparison with markers of cell proliferation and neuronal differentiation.
Ferreiro-Galve S, Rodríguez-Moldes I, Candal E. J Exp Zool B Mol Dev Evol. 2012 Mar; 318(2):91-108.
Calretinin immunoreactivity in the developing retina of sharks: comparison with cell proliferation and GABAergic system markers.
Exp Eye Res. 2010
Calretinin immunoreactivity in the developing retina of sharks: comparison with cell proliferation and GABAergic system markers.
Ferreiro-Galve S, Rodríguez-Moldes I, Candal E. Exp Eye Res. 2010 Sep; 91(3):378-86. Epub 2010 Jun 19.
Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration.
Exp Eye Res. 2008
Characterization of Müller glia and neuronal progenitors during adult zebrafish retinal regeneration.
Thummel R, Kassen SC, Enright JM, Nelson CM, Montgomery JE, Hyde DR. Exp Eye Res. 2008 Nov; 87(5):433-44. Epub 2008 Aug 5.
Dynamic Pax6 expression during the neurogenic cell cycle influences proliferation and cell fate choices of retinal progenitors.
Neural Dev. 2009
Dynamic Pax6 expression during the neurogenic cell cycle influences proliferation and cell fate choices of retinal progenitors.
Hsieh YW, Yang XJ. Neural Dev. 2009 Aug 17; 4:32. Epub 2009 Aug 17.
Development of the cerebellar body in sharks: spatiotemporal relations of Pax6 expression, cell proliferation and differentiation.
Neurosci Lett. 2008
Development of the cerebellar body in sharks: spatiotemporal relations of Pax6 expression, cell proliferation and differentiation.
Rodríguez-Moldes I, Ferreiro-Galve S, Carrera I, Sueiro C, Candal E, Mazan S, Anadón R. Neurosci Lett. 2008 Feb 20; 432(2):105-10. Epub 2007 Dec 7.

To top • Home

TraveldoctorOnline 2001 • Disclaimer • webmaster

The contents within traveldoctoronline are presented only for informational purposes and cannot substitute for professional health care or any other medical treatment.All users of this website with health problems should be oblige always to consult their medical doctor before starting any treatment.