Authors:
Address: Dr Safia Sultana, Thesis Part Student of M Phil Microbiology, Mymensingh Medical College (MMC), Mymensingh, Bangladesh.
Journal:

Publication:

abstract

typhoid Fever, caused by Salmonella typhi, is an important cause of morbidity and mortality in many developing countries. A rapid and sensitive method for the detection of S. typhi is essential for early diagnosis. This was a study to prospectively evaluate the sensitivity and specificity of Nested polymerase chain reaction (PCR) to identify the S. typhi using flagellin gene related primers. The study was carried out in the department of Microbiology, Mymensingh Medical College, Mymensingh between July, 2010 and June, 2011, including 82 individuals of different age and sex. Of them, 62 were clinically suspected cases of typhoid fever and remaining 20 were apparently healthy controls. Cultures as well as PCR of blood specimens were performed for each of the cases. Among the 62 suspected typhoid fever cases, 8(12.9%) were blood culture positive and 55(88.7%) were PCR positive for S. typhi. All culture positive cases were positive by PCR and among 54 culture negative cases, 47(87%) were positive by PCR. Neither of the healthy controls was positive by PCR or blood culture. The sensitivity, specificity, positive predictive value and negative predictive value of PCR using blood culture as gold standard were 88.7%, 100%, 100% and 74% respectively for typhoid fever. In this study, the PCR appears highly specific, very sensitive and superior to blood culture for the early diagnosis of typhoid fever.

Related Articles
Diagnosis of typhoid fever by polymerase chain reaction.
Indian J Pediatr. 2007
Diagnosis of typhoid fever by polymerase chain reaction.
Ambati SR, Nath G, Das BK. Indian J Pediatr. 2007 Oct; 74(10):909-13.
Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi.
J Infect Chemother. 2003
Rapid diagnosis of typhoid fever by PCR assay using one pair of primers from flagellin gene of Salmonella typhi.
Massi MN, Shirakawa T, Gotoh A, Bishnu A, Hatta M, Kawabata M. J Infect Chemother. 2003 Sep; 9(3):233-7.
A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi.
Ann Clin Microbiol Antimicrob....
A fast and highly sensitive blood culture PCR method for clinical detection of Salmonella enterica serovar Typhi.
Zhou L, Pollard AJ. Ann Clin Microbiol Antimicrob. 2010 Apr 19; 9:14. Epub 2010 Apr 19.
Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction.
J Clin Microbiol. 1993
Detection of Salmonella typhi in the blood of patients with typhoid fever by polymerase chain reaction.
Song JH, Cho H, Park MY, Na DS, Moon HB, Pai CH. J Clin Microbiol. 1993 Jun; 31(6):1439-43.
Detection of Salmonella typhi by polymerase chain reaction: implications in diagnosis of typhoid fever.
Infect Genet Evol. 2002
Detection of Salmonella typhi by polymerase chain reaction: implications in diagnosis of typhoid fever.
Kumar A, Arora V, Bashamboo A, Ali S. Infect Genet Evol. 2002 Dec; 2(2):107-10.

To top • Home

---

Show map | Diseases | Vaccination | Chronic disease | Medicine | Pregnancy | Heat & Sunburn | Cold | Security | Useful tips | Faq | News

TraveldoctorOnline 2001 • Disclaimer • webmaster