Identification of a 24 kDa excretory secretory protein in Anisakis simplex.

Authors:
Address: Department of Urology, School of Medicine, Pusan National University, Yangsan-si, Gyeongsannam-do 626-870, Republic of Korea.
Journal:

Publication:

abstract

A gene coding for a 24 kDa protein (22 U homologous; As22U) was isolated from the Anisakis simplex third-stage larvae cDNA library during expressed sequence tag analysis. As22U was 636 bp long, and was found to code for 212 amino acid residues with a calculated mass of 23.5 kDa and a PI of 9.06. The As22U deduced amino acid sequence harbored a signal peptide region and 16 highly conserved cysteine residues, and it was identified in both the total extracts and excretory secretory (ES) protein of A. simplex. Its molecular weight was measured at 24 kDa via western blot analysis. The expression levels of thymic stromal lymphopoietin, IL-25, and CXCL1 (Gro-α) genes were increased at 6h after recombinant As22U treatment in mouse intestinal epithelial cells. Additionally, thymus and activation-regulated chemokine gene levels were increased at 14 h after treatment. Although we do not currently have sufficient evidence to determine whether As22U plays a role as an allergen, this remains possible. Further in vivo studies may provide some insight as to the allergenic properties of As22U.

Related Articles
A recombinant enolase from Anisakis simplex is differentially recognized in natural human and mouse experimental infections.
Med Microbiol Immunol. 2006
A recombinant enolase from Anisakis simplex is differentially recognized in natural human and mouse experimental infections.
Rodríguez E, Romarís F, Lorenzo S, Moreno J, Bonay P, Ubeira FM, Gárate T. Med Microbiol Immunol. 2006 Mar; 195(1):1-10. Epub 2005 Jul 28.
Purification and molecular cloning of a major allergen from Anisakis simplex.
Mol Biochem Parasitol. 2004
Purification and molecular cloning of a major allergen from Anisakis simplex.
Shimakura K, Miura H, Ikeda K, Ishizaki S, Nagashima Y, Shirai T, Kasuya S, Shiomi K. Mol Biochem Parasitol. 2004 May; 135(1):69-75.
Purification and cDNA cloning of a new heat-stable allergen from Anisakis simplex.
Mol Biochem Parasitol. 2007
Purification and cDNA cloning of a new heat-stable allergen from Anisakis simplex.
Kobayashi Y, Shimakura K, Ishizaki S, Nagashima Y, Shiomi K. Mol Biochem Parasitol. 2007 Oct; 155(2):138-45. Epub 2007 Jun 30.
Cloning and characterisation of the Anisakis simplex allergen Ani s 4 as a cysteine-protease inhibitor.
Int J Parasitol. 2007
Cloning and characterisation of the Anisakis simplex allergen Ani s 4 as a cysteine-protease inhibitor.
Rodriguez-Mahillo AI, Gonzalez-Muñoz M, Gomez-Aguado F, Rodriguez-Perez R, Corcuera MT, Caballero ML, Moneo I. Int J Parasitol. 2007 Jul; 37(8-9):907-17. Epub 2007 Jan 27.
A 24 kDa excretory-secretory protein of Anisakis simplex larvae could elicit allergic airway inflammation in mice.
Korean J Parasitol. 2011
A 24 kDa excretory-secretory protein of Anisakis simplex larvae could elicit allergic airway inflammation in mice.
Park HK, Cho MK, Park MK, Kang SA, Kim YS, Kim KU, Lee MK, Ock MS, Cha HJ, Yu HS. Korean J Parasitol. 2011 Dec; 49(4):373-80. Epub 2011 Dec 16.
A 24 kDa excretory-secretory protein of Anisakis simplex larvae could elicit allergic airway inflammation in mice.
Korean J Parasitol. 2011
A 24 kDa excretory-secretory protein of Anisakis simplex larvae could elicit allergic airway inflammation in mice.
Park HK, Cho MK, Park MK, Kang SA, Kim YS, Kim KU, Lee MK, Ock MS, Cha HJ, Yu HS. Korean J Parasitol. 2011 Dec; 49(4):373-80. Epub 2011 Dec 16.

To top • Home

TraveldoctorOnline 2001 • Disclaimer • webmaster

The contents within traveldoctoronline are presented only for informational purposes and cannot substitute for professional health care or any other medical treatment.All users of this website with health problems should be oblige always to consult their medical doctor before starting any treatment.