Exploiting the Campylobacter jejuni protein glycosylation system for glycoengineering vaccines and diagnostic tools directed against brucellosis.

Authors:
Address: Department of Biological Sciences, University of Alberta, Edmonton, AB, Canada.
Journal:

Publication:
Free Text: Exploiting the Campylobacter jejuni protein glycosylation system for glycoengineering vaccines and diagnostic tools directed against brucellosis.

abstract

BACKGROUND:

Immune responses directed towards surface polysaccharides conjugated to proteins are effective in preventing colonization and infection of bacterial pathogens. Presently, the production of these conjugate vaccines requires intricate synthetic chemistry for obtaining, activating, and attaching the polysaccharides to protein carriers. Glycoproteins generated by engineering bacterial glycosylation machineries have been proposed to be a viable alternative to traditional conjugation methods.

RESULTS:

In this work we expressed the C. jejuni oligosaccharyltansferase (OTase) PglB, responsible for N-linked protein glycosylation together with a suitable acceptor protein (AcrA) in Yersinia enterocolitica O9 cells. MS analysis of the acceptor protein demonstrated the transfer of a polymer of N-formylperosamine to AcrA in vivo. Because Y. enterocolitica O9 and Brucella abortus share an identical O polysaccharide structure, we explored the application of the resulting glycoprotein in vaccinology and diagnostics of brucellosis, one of the most common zoonotic diseases with over half a million new cases annually. Injection of the glycoprotein into mice generated an IgG response that recognized the O antigen of Brucella, although this response was not protective against a challenge with a virulent B. abortus strain. The recombinant glycoprotein coated onto magnetic beads was efficient in differentiating between naïve and infected bovine sera.

CONCLUSION:

Bacterial engineered glycoproteins show promising applications for the development on an array of diagnostics and immunoprotective opportunities in the future.

Related Articles
Desulfovibrio desulfuricans PglB homolog possesses oligosaccharyltransferase activity with relaxed glycan specificity and distinct protein acceptor sequence requirements.
Glycobiology. 2011
Desulfovibrio desulfuricans PglB homolog possesses oligosaccharyltransferase activity with relaxed glycan specificity and distinct protein acceptor sequence requirements.
Ielmini MV, Feldman MF. Glycobiology. 2011 Jun; 21(6):734-42. Epub 2010 Nov 22.
Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli.
Proc Natl Acad Sci U S A. 2005
Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli.
Feldman MF, Wacker M, Hernandez M, Hitchen PG, Marolda CL, Kowarik M, Morris HR, Dell A, Valvano MA, Aebi M. Proc Natl Acad Sci U S A. 2005 Feb 22; 102(8):3016-21. Epub 2005 Feb 9.
Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 II the use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants.
Vet Microbiol. 1995
Serological crossreactivity between Brucella abortus and Yersinia enterocolitica 0:9 II the use of Yersinia outer proteins for the specific detection of Yersinia enterocolitica infections in ruminants.
Kittelberger R, Hilbink F, Hansen MF, Ross GP, Joyce MA, Fenwick S, Heesemann J, Wolf-Watz H, Nielsen K. Vet Microbiol. 1995 Dec; 47(3-4):271-80.
Review Brucellosis vaccines: past, present and future.
Vet Microbiol. 2002
Review Brucellosis vaccines: past, present and future.
Schurig GG, Sriranganathan N, Corbel MJ. Vet Microbiol. 2002 Dec 20; 90(1-4):479-96.
Review Rough vaccines in animal brucellosis: structural and genetic basis and present status.
Vet Res. 2004
Review Rough vaccines in animal brucellosis: structural and genetic basis and present status.
Moriyón I, Grilló MJ, Monreal D, González D, Marín C, López-Goñi I, Mainar-Jaime RC, Moreno E, Blasco JM. Vet Res. 2004 Jan-Feb; 35(1):1-38.

To top • Home

TraveldoctorOnline 2001 • Disclaimer • webmaster

The contents within traveldoctoronline are presented only for informational purposes and cannot substitute for professional health care or any other medical treatment.All users of this website with health problems should be oblige always to consult their medical doctor before starting any treatment.