Evaluation of a Rapid Immunochromatographic Diagnostic Test for the detection of rabies from brain material of European mammals.

Authors:
Address: French Agency for Food, Environmental and Occupational Health Safety (Anses), Nancy Laboratory for Rabies and Wildlife, WHO Collaborating Centre for Research and Management in Zoonoses Control, Technopôle agricole et vétérinaire, Domaine de Pixérécourt, BP40009, 54220 Malzéville, France. alexandre.servat@anses.fr
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abstract

the surveillance of rabies relies on investigations conducted on dead suspected animals or animals showing clinical signs suggestive of rabies. An Immunochromatographic method based on lateral flow principle has been evaluated against a collection of brain samples mainly of European mammals including bats. The performance of this new Test has been compared to the conventional gold standard methods: the fluorescent Antibody Test (FAT) and the Rapid Tissue Culture Infection Test (RTCIT). This test enabled the detection of various rabies strains belonging to rabies species 1, 5, 6 and 7 and demonstrated an overall specificity of 100% and a sensitivity of more than 88% when compared to FAT and RTCIT. A total agreement between the Rapid Immunochromatographic Diagnostic Test and conventional technique results have been obtained for European bat samples.

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