Effects of resinous monomers on the odontogenic differentiation and mineralization potential of highly proliferative and clonogenic cultured apical papilla stem cells.

Authors:
Address: Department of Fixed Prosthesis & Implant Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki GR-54124, Greece.
Journal:

Publication:

abstract

OBJECTIVE:

the aim of this study was to investigate the Effects of resinous monomers on the odontogenic differentiation and mineralization potential of apical papilla stem cells (SCAP).

METHODS:

Cultures were established from developing third molars of healthy donors aged 14-18 years-old and were extensively characterized for proliferation rate, colony forming unit efficiency and expression of stem cell markers (STRO-1, CD146, CD34, CD45, CD105, CD117-c-Kit, CD24, CD90, Nanog, Oct3/4), in order to select those with enhanced stem cell and odontogenic differentiation properties. SCAP enriched cultures were then induced for odontogenic differentiation in the continuous presence of low concentrations (0.05-0.5 mM) of the monomers 2-hydroxy-ethyl-methacrylate-HEMA and triethylene-glycol-dimethacrylate-TEGDMA for 3 weeks (long-term exposure). Additionally, the effects of a single exposure (72 h) to higher concentrations of HEMA (2 mM) and TEGDMA (1 mM) were evaluated.

RESULTS:

The results showed that both types of monomer-exposure significantly delayed the odontogenic differentiation and mineralization processes of SCAP cells. A down-regulation followed by recovery in the expression of differentiation markers, including dentin sialophosphoprotein-DSPP, bone sialoprotein-BSP, osteocalcin-OCN and alkaline phosphatase-ALP was recorded. This was accompanied by reduction of the mineralized matrix produced by monomer-treated-compared to non-treated contol cultures. Furthermore, a concentration-dependence was observed for both monomers during long-term exposure, whereas the effects of HEMA were evident at much lower concentrations compared to TEGDMA.

SIGNIFICANCE:

These findings suggest that resinous monomers can delay the odontogenic differentiation of SCAP cells, potentially disturbing the physiological repair and/or developmental processes of human permanent teeth.

Related Articles
Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP).
Arch Oral Biol. 2011
Comparative analysis of in vitro osteo/odontogenic differentiation potential of human dental pulp stem cells (DPSCs) and stem cells from the apical papilla (SCAP).
Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W. Arch Oral Biol. 2011 Jul; 56(7):709-21. Epub 2011 Jan 11.
Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla.
J Endod. 2012
Basic fibroblast growth factor enhances stemness of human stem cells from the apical papilla.
Wu J, Huang GT, He W, Wang P, Tong Z, Jia Q, Dong L, Niu Z, Ni L. J Endod. 2012 May; 38(5):614-22. Epub 2012 Feb 29.
Assessment of the impact of two different isolation methods on the osteo/odontogenic differentiation potential of human dental stem cells derived from deciduous teeth.
Calcif Tissue Int. 2011
Assessment of the impact of two different isolation methods on the osteo/odontogenic differentiation potential of human dental stem cells derived from deciduous teeth.
Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W. Calcif Tissue Int. 2011 Feb; 88(2):130-41. Epub 2010 Dec 14.
Review Genetic and cellular toxicology of dental resin monomers.
J Dent Res. 2006
Review Genetic and cellular toxicology of dental resin monomers.
Schweikl H, Spagnuolo G, Schmalz G. J Dent Res. 2006 Oct; 85(10):870-7.
Review Differentiation potential of dental papilla, dental pulp, and apical papilla progenitor cells.
J Endod. 2010
Review Differentiation potential of dental papilla, dental pulp, and apical papilla progenitor cells.
Tziafas D, Kodonas K. J Endod. 2010 May; 36(5):781-9. Epub 2010 Mar 24.

To top • Home

TraveldoctorOnline 2001 • Disclaimer • webmaster

The contents within traveldoctoronline are presented only for informational purposes and cannot substitute for professional health care or any other medical treatment.All users of this website with health problems should be oblige always to consult their medical doctor before starting any treatment.