Analysis of the Complete Genome of Tembusu Virus, a Flavivirus Isolated from Ducks in China.

Authors: Tang Y Diao Y Gao X Yu C Chen L Zhang D
Address: Zoology Institute, Agricultural University of Shan Dong province, Shan Dong province, China Key Laboratory of Zoonosis of Ministry, China Agricultural University, Beijing city, China.
Journal:

Publication:

abstract

During investigations into the outbreak of duck viral infection in 2010 in China, with a severe drop in egg production, a flaviVirus was Isolated from the affected Ducks. It was characterized as a Tembusu virus (TMUV). In this study, we obtained a Complete Genome sequence of Tembusu virus using RT-PCR and RACE techniques. TMUV genome is a singled-stranded RNA, with 10 990 nucleotides in length, and contains a single open reading frame (3410 amino acids) encoding 11 viral proteins with 5'and 3'non-translated regions (NTRs) of 142 and 618 nt, respectively. We characterized the open reading frame (ORF) with respect to gene sizes, cleavage sites and potential glycosylation sites. The different genomic regions of the virus were also compared with those of six other Flaviviruses including Japanese encephalitis virus, West Nile virus (WNV), dengue-2 virus, yellow fever virus, tick-borne encephalitis virus (TBEV) and Bagaza virus. TMUV demonstrated the highest similarity to Bagaza virus. The result of entire ORF scanning shows that TMUV was close to Bagaza viruses in genetic relatedness. These data demonstrate that TMUV is a unique virus among the mosquito-borne flaviviruses and also provide a useful reference for a critically important study to determine why TMUV is a serious pathogen for ducks.

Related Articles
Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus.
PLoS One. 2011
Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus.
Su J, Li S, Hu X, Yu X, Wang Y, Liu P, Lu X, Zhang G, Hu X, Liu D, et al. PLoS One. 2011 Mar 24; 6(3):e18106. Epub 2011 Mar 24.
Genetic characterization of Yokose virus, a flavivirus isolated from the bat in Japan.
Virology. 2005
Genetic characterization of Yokose virus, a flavivirus isolated from the bat in Japan.
Tajima S, Takasaki T, Matsuno S, Nakayama M, Kurane I. Virology. 2005 Feb 5; 332(1):38-44.
Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses.
Arch Virol. 2007
Full-length sequencing and genomic characterization of Bagaza, Kedougou, and Zika viruses.
Kuno G, Chang GJ. Arch Virol. 2007; 152(4):687-96. Epub 2007 Jan 3.
Review The molecular biology of tick-borne encephalitis virus. Review article.
APMIS. 1993
Review The molecular biology of tick-borne encephalitis virus. Review article.
Heinz FX, Mandl CW. APMIS. 1993 Oct; 101(10):735-45.
Review Dengue and other emerging flaviviruses.
J Infect. 2001
Review Dengue and other emerging flaviviruses.
Solomon T, Mallewa M. J Infect. 2001 Feb; 42(2):104-15.

To top • Home

TraveldoctorOnline 2001 • Disclaimer • webmaster

The contents within traveldoctoronline are presented only for informational purposes and cannot substitute for professional health care or any other medical treatment.All users of this website with health problems should be oblige always to consult their medical doctor before starting any treatment.